Effect of Maize and Oyster Mushroom Blending Ratio on Nutritional and Sensory Quality of Maize-Mushroom Composite

Effect of Maize and Oyster Mushroom Blending Ratio on Nutritional and Sensory Quality of Maize-Mushroom Composite

Godfrey Kilama Docus Alowo Simon Zziwa Solomon Olum

Department of Food Innovation and Nutrition, Faculty of Agriculture and Environment, Mountains of the Moon University, P.O. Box 837, Fort Portal, Uganda

Department of Food Science and Post-harvest Technology, Faculty of Agriculture and Environment, Gulu University, P.O. Box 166, Gulu, Uganda

*Corresponding author; E-mail: kilamagodfrey632@gmail.com

Abstract

Background: Oyster mushroom (Pleurotus ostreatus), though underutilized in complementary feeding, is rich in micronutrients. Blending it with maize flour can improve the nutritional quality of cereal-based foods widely used in resource-constrained settings, though its composite effects remain largely unexplored.

Problem Statement: Despite ongoing efforts, iron and zinc deficiencies remain prevalent among infants in Northern Uganda due to reliance on maize-based complementary foods. Promoting home-based traditional diets using local sources like oyster mushrooms offers a nutrient-rich alternative. This study examined maize and mushroom composites.

Study Overview
5
Blending ratios tested
65
Caregivers for sensory evaluation
10%
Optimal mushroom inclusion
6-24
Target infant age (months)

Methodology

Sample Preparation:

  • Quality maize and fresh oyster mushrooms purchased from Gulu District
  • Dried, sorted, cleaned, and milled into flour

Blending Ratios (w/w):

  • 1:0 (100% maize)
  • 9:1 (90% maize, 10% mushroom)
  • 8:2 (80% maize, 20% mushroom)
  • 7:3 (70% maize, 30% mushroom)
  • 6:4 (60% maize, 40% mushroom)

Analysis Methods: Proximate and mineral composition using AOAC 2005 methods · Sensory evaluation with 65 caregivers

Results

Increased Nutrients

Significant increases (p < 0.05) with higher mushroom ratios:

  • Protein: 8.74% → 17.87%
  • Fiber: 0.76% → 3.58%
  • Ash: 1.14% → 3.13%
  • Zinc: 1.1% → 3.4%
  • Iron: 2.3% → 8.2%
  • Calcium: 28% → 55%
  • Magnesium: 19% → 53%

Decreased Nutrients & Sensory

Declined with higher mushroom ratios:

  • Moisture: 4.67% → 3.13%
  • Fat: 1.45% → 1.29%
  • Carbohydrate: 83.25% → 70.77%
  • Sensory scores: Declined
Key finding: Acceptability at 10% inclusion matched maize-only flour (p > 0.05)

Nutritional Comparison

Parameter 100% Maize 10% Mushroom 20% Mushroom 30% Mushroom 40% Mushroom
Protein (%) 8.74 10.57 12.40 15.13 17.87
Fiber (%) 0.76 1.47 2.18 2.88 3.58
Iron (%) 2.3 3.5 4.8 6.5 8.2
Zinc (%) 1.1 1.6 2.1 2.8 3.4
Carbohydrate (%) 83.25 80.13 76.87 73.52 70.77

Conclusion

Blending maize flour with oyster mushroom significantly improves protein, fiber, and key micronutrients while maintaining sensory acceptability at 10% inclusion. This innovation supports complementary feeding, informs nutrition policy, and strengthens household food security, offering a sustainable solution to micronutrient deficiencies in infants 6-24 months.

Children Maize-mushroom composite Micronutrient deficiency Nutritional quality Sensory quality Uganda
2025 Uganda